Oregon spotted frog – endangered in British Columbia

Published source details Thoney D.A. (2011) Oregon spotted frog – endangered in British Columbia. Amphibian Ark Newsletter, 17, 13.
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This study is summarised as evidence for the following.

	Action	Category
	Amphibians: Use hormone treatment to induce sperm and egg release Action Link
	Use hormone treatment to induce sperm and egg release during captive breeding Action Link

Amphibians: Use hormone treatment to induce sperm and egg release
Use hormone treatment to induce sperm and egg release during captive breeding

A replicated study in 2010–2011 of captive Oregon spotted frogs Rana pretiosa in Vancouver, Canada (Thoney 2011) found that frogs bred successfully in captivity and that treatment with hormones did not increase the proportion of females producing eggs or numbers of eggs. The two hormonal substances tested did synchronize timing of egg production. The small number of mature frogs produced 291 tadpoles in the first year. In 2011, a larger number of frogs bred and over 9,000 eggs were produced, of which 3,000 hatched. Providing a seasonal daylight and temperature regime was considered by the authors to be crucial to breeding success. Metamorphs and tadpoles were released in spring 2011. Eggs were collected each year from the wild to increase genetic diversity of the captive population.