Husbandry and breeding of the Puerto-Rican toad (Peltophryne lemur) with comments on its natural history

Published source details Miller T.J. (1985) Husbandry and breeding of the Puerto-Rican toad (Peltophryne lemur) with comments on its natural history. Zoo Biology, 4, 281-286.
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This study is summarised as evidence for the following.

	Action	Category
	Amphibians: Use hormone treatment to induce sperm and egg release Action Link
	Use hormone treatment to induce sperm and egg release during captive breeding Action Link

Amphibians: Use hormone treatment to induce sperm and egg release
Use hormone treatment to induce sperm and egg release during captive breeding

A replicated study in 1983 of Puerto Rican toads Peltophryne lemur at Buffalo Zoological Gardens, USA (Miller 1985) found that following hormone treatment, one of three females produced viable eggs. Another female produced hundreds of infertile eggs and the third a few unfertilized eggs. Over 150 tadpoles hatched from the viable clutch and tadpole survival was 100%. In 1983, 75 of the toadlets were released at an artificial pond in Puerto Rico. Three male and female captive bred-toads were housed in an enclosure. Breeding was induced by lutenizing hormone-releasing hormone (0.01–0.05 ml/10 g body weight). Tadpoles were transferred to aquaria for rearing and metamorphs to containers.