Hormonal induction of gamete release and in-vitro fertilisation in the critically endangered southern corroboree frog, Pseudophryne corroboree

Published source details Byrne P.G. & Silla A.J. (2010) Hormonal induction of gamete release and in-vitro fertilisation in the critically endangered southern corroboree frog, Pseudophryne corroboree. Reproductive Biology and Endocrinology, 8, 144.
Share Tweet Email

Actions

This study is summarised as evidence for the following.

	Action	Category
	Amphibians: Use artificial fertilization in captive breeding Action Link
	Use artificial fertilization in captive breeding Action Link
	Amphibians: Use hormone treatment to induce sperm and egg release Action Link
	Use hormone treatment to induce sperm and egg release during captive breeding Action Link

Amphibians: Use artificial fertilization in captive breeding
Use artificial fertilization in captive breeding

A replicated study in 2009 of southern corroboree frogs Pseudophryne corroboree at Monash University, Australia (Byrne & Silla 2010) found that artificial fertilization resulted in 55% of eggs being fertilized, but embryos failed prior to gastrulation. Fertilization and the stage that the embryo failed varied between and within females. Hormone treatment was used to induce sperm and egg release. Artificial fertilization was attempted by combining spermic urine (1.1–2.9 x 10²) with eggs from five females in a dilute solution of simplified amphibian Ringer solution at 10°C. Embryonic development was checked every 6–12 hours for seven days.
Amphibians: Use hormone treatment to induce sperm and egg release
Use hormone treatment to induce sperm and egg release during captive breeding

A randomized, replicated, controlled study in 2009 of southern corroboree frogs Pseudophryne corroboree at Monash University, Australia (Byrne & Silla 2010) found that hormone treatment successfully induced sperm release and to a lesser extent egg production. Human chorionic gonadotropin (hCG) and luteinizing hormone-releasing hormone (LHRHa) both induced significantly higher proportions of males to release sperm than controls (82 vs 0%). LHRHa treated males released significantly higher numbers of sperm (670 vs 50) and concentration of sperm (4,500 vs 800 x 103/ml) over a longer period than those treated with human chorionic gonadotropin. There was no significant difference in numbers of females releasing eggs following LHRHa and controls (30 vs 0%). Eggs were released 24–48 hours post-treatment (peak 36 hours). Average clutch size was 15. Six randomly selected males were given a dose of either 20 μg/g bodyweight of human chorionic gonadotropin or 5 μg/g of LHRHa in simplified amphibian Ringer solution (SAR) or a control of 0.1 ml of SAR. Sperm response was tested in urine seven times up to 72 hours post-treatment. Seventeen females received a priming (1 μg/g) and ovulatory dose (5 μg/g) of LHRHa in SAR. Eight received a control of 0.1 ml of SAR. Ovulation was tested every 12 hours for five days.