Producing progeny from endangered birds of prey: treatment of urine-contaminated semen and a novel intramagnal insemination approach
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Published source details
Blanco J.M., Gee G.F., Wildt D.E. & Donoghue A.M. (2002) Producing progeny from endangered birds of prey: treatment of urine-contaminated semen and a novel intramagnal insemination approach. Journal of Zoo and Wildlife Medicine, 33, 1-7.
Published source details Blanco J.M., Gee G.F., Wildt D.E. & Donoghue A.M. (2002) Producing progeny from endangered birds of prey: treatment of urine-contaminated semen and a novel intramagnal insemination approach. Journal of Zoo and Wildlife Medicine, 33, 1-7.
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This study is summarised as evidence for the following.
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Wash contaminated semen and use it for artificial insemination Action Link |
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Wash contaminated semen and use it for artificial insemination
A replicated controlled study from February-April in a captive breeding programme in Spain (Blanco et al. 2002) found that urine-contaminated sperm can be used to artificially inseminate raptor females after washing the sperm with an alkalinised diluent. Urine contamination of ejaculate samples was high in all 4 species (25 individuals) analysed (37% for golden eagle Aquila chrysaetos, 43% for Spanish imperial eagle Aquila adalberti, 29% for Bonelli’s eagle A. fasciatus (also Hieraaetus fasciatus) and 48% for peregrine falcon Falco peregrinus) and significantly reduced semen pH (6.5-6.9 compared to 7.2-7.6). However, sperm motility was significantly higher in sperm washed with an alkalinised diluent (compared to a neutral diluent). An intramagnal insemination technique of washed semen produced 1 golden eagle and 2 peregrine falcon nestlings (11 and 16% of clutch size respectively). Each sperm sample was divided into two equal amounts and washed with either neutral (pH 7.0) or alkalinised (pH 8.0) diluent (Lake’s formula with 300 mg / 100 ml of citric acid added) before being incubated for 30 min (21°C).
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